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KMID : 0368419920350010009
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Journal of Plant Biology
1992 Volume.35 No. 1 p.9 ~ p.15
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Changes in Isozyme Patterns of Peroxidase and Esterase during Regeneration of Pepper(Capsicum annuum L.)
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鄭賢淑/Cheong, Hyeon Sook
林仙禧/金玲希/康榮熹/Lim, Sun Hee/Kim, Young Hee/Kang, Young Hee
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Abstract
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The plantlet was regenerated on MS medium containing BAP (2 ㎎/ℓ) and IAA (1 ㎎/ℓ) from leaf discs of pepper after 3 weeks of culture. And then, we investigated the activity of peroxidase and esterase and the pattern of their isozymes from leaf, stem and root in order to observe physiological and biochemical changes of the devlopemental stage, respectively. The peroxidase was expressed with tissue specificity because peroxidase activity according to the developemental stage of the tissue was not only highest in the leaf of the pepper at 10 days after it germinated but also 2 new bands of its isozyme were found in pI 7.2 and pI 5.2. However, a new pI 3.4 band was found in the leaf and root of the pepper after 14 days of germination, and in the stem was found out pI 5.2 band. As regeneration of leaf dises was progressed, its peroxiase activity was increased about 80% more than that of control after 14 days of culture and new pI 3.2 and 6.5 bands of it isozyme were found. The results suggested that peroxidase would be connected with regeneration of pepper. Also, esterase activity was increased about 50% more than that of control after 14 days of culture, the pattern of esterase isozyme was shown to be 3 cathodic bands and 1 anodic band after 7 days of culture.
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